Compatible-Solute-Supported Periplasmic Expression of Functional Recombinant Proteins under Stress Conditions
نویسندگان
چکیده
منابع مشابه
Glucosylglycerol, a compatible solute, sustains cell division under salt stress.
The cyanobacterium Synechocystis sp. PCC 6803 accumulates the compatible solute glucosylglycerol (GG) and sucrose under salt stress. Although the molecular mechanisms for GG synthesis including regulation of the GG-phosphate synthase (ggpS) gene, which encodes GgpS, has been intensively investigated, the role of GG in protection against salt stress remains poorly understood. In our study of the...
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In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the related cDNA was inserted in two expression plasmids carrying pelB signal peptide, one with lac bacterial promoter and the other with a bacteriophage T7-based promoter. The recombinant plasmids were moved to TG1 and BL21 strains of E. coli, respectively. To induce the expression systems, IPTG and...
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Salinity tolerance of four grape genotypes [GharaUzum, Hosseini, AghUzum and Keshmeshi] was studied under various salinity levels (25, 50 and 100 mM NaCl). As a result, growth indices were significantly (P<0.05) reduced by salinity, whereas Cl- and Na+ contents in the plant parts were increased. Cl- accumulation exceeded than that of Na+ in all treatments. Among the genotypes studied, GharaUzum...
متن کاملconstruction of recombinant plasmids for periplasmic expression of human growth hormone in escherichia coli under t7 and lac promoters
in order to study the periplasmic expression of human growth hormone (hgh) in escherichia coli, the related cdna was inserted in two expression plasmids carrying pelb signal peptide, one with lac bacterial promoter and the other with a bacteriophage t7-based promoter. the recombinant plasmids were moved to tg1 and bl21 strains of e. coli, respectively. to induce the expression systems, iptg and...
متن کاملRegulation of compatible solute accumulation in bacteria.
In their natural habitats, microorganisms are often exposed to osmolality changes in the environment. The osmotic stress must be sensed and converted into an activity change of specific enzymes and transport proteins and/or it must trigger their synthesis such that the osmotic imbalance can be rapidly restored. On the basis of the available literature, we conclude that representative gram-negat...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2000
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.66.4.1572-1579.2000